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KMID : 1377020200170030285
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Tissue Engineering and Regenerative Medicine
2020 Volume.17 No. 3 p.285 ~ p.299
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Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering
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Mallis Panagiotis
Katsimpoulas Michalis
Kostakis Alkiviadis
Dipresa Daniele
Korossis Sotiris
Papapanagiotou Aggeliki
Kassi Eva
Stavropoulos-Giokas Catherine
Michalopoulos Efstathios
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Abstract
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Background: The development of a biological based small diameter vascular graft (d?
Methods: Human UAs were decellularized using 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) detergents. Then, vitrified with vitrification solution 55 (VS55) solution, remained for 6 months in liquid nitrogen and their extracellular matrix composition was compared to conventionally cryopreserved UAs. Additionally, total hydroxyproline, sulphated glycosaminoglycan and DNA content were quantified in all samples. Finally, the vitrified umbilical arteries implanted as common carotid artery interposition graft to a porcine animal model.
Results: Decellularized and vitrified UAs characterized by proper preservation of extracellular matrix proteins and tissue architecture, whereas conventionally cryopreserved samples exhibited a disorganized structure. Total hydroxyproline content was preserved, although sulphated glycosaminoglycan and DNA contents presented significantly alterations in all samples. Implanted UAs successfully recellularized and remodeled as indicated by the histological analysis.
Conclusion: Decellularized and vitrified UAs retained their structure function properties and can be possible used as an alternative source for readily accessible small diameter vascular grafts.
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KEYWORD
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Human umbilical arteries, Decellularization, Vitrification, Hydroxyproline quantification, Vascular graft, Carotid artery
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